Solid-phase extraction derivative aromatic compounds in water samples and determination of using GC-FID

A simple method to pre-concentrated derivative aromatic compounds on active carbon in column has been applied as stationary phase which is used to measure the concentration of derivative aromatic compounds in water samples by means of solid-phase extraction. To measure 250 cc water samples and 250mg active carbon could be applied. Next step is to measure the derivative aromatic compounds by injecting them to the gas chromatography with flame ionization. The advantages of applying gas chromatography–flame ionization detection (GC–FID) with SPE in presence Carbon active are high sensitivity, High speed transformation of aromatic compounds and improving ration standard for river waters with derivative aromatic compounds in the range of ppb or those with less than 10% of LOD. The quantity of extraction could be affected by sample’s pH, amount of solvent, washing liquid type, solvent and flow rates of the sample solutions. Key word: Solid phase extraction, Water analyzing, active carbon, derivative aromatic (phenol compounds), Gas chromatography flame ionization detection (GC–FID). The most common techniques for the analysis of anilines and phenols in environmental waters are gas (GC) and high-performance liquid chromatography (HPLC)1. The direct analysis of phenols by GC is difficult4 and GC analysis is usually performed after a derivatization step1,5-7. Anilines are also thermolabile and polar compounds and a derivatization step is often required before GC analysis3,8-11. Most of the derivatization processes however are not straightforward and sometimes require handling of hazardous chemicals. Since in HPLC analysis there are no derivatization requirements, it appears to be a good alternative to GC analysis and nowadays has been widely accepted as the method of choice1,12. A large number of procedures for the determination of phenolic compounds in water using SPE have been tested13,14,18-20. Various types of solidphase sorbents have been used, including C18 21,22, polystyrenedivinylbenzene-based polymers22,23, and various forms of carbon18,20. A number of these sorbents show relatively low recovery for some phenolic compounds13,14,7,24,25.In this study, a βcyclodextrinbounded silica on active carbon in CBP column has been applied as stationary phase which is used to measure the concentration of derivative aromatics (phenol compounds) in water samples by means of solid-phase extraction.